人IL-1 beta ELISA试剂盒(without plates)| Abcam中文官网-ChromoTek公司新闻-蚂蚁淘厂家直采,部分现货.

产品概述

Abcam s IL-1 Human in vitro ELISA Set is designed for the quantitative measurement of IL-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

ThisELISA Set is intended to measure human IL-1b in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The IL-1b ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human IL-1b.

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
翻译后修饰 Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
细胞定位 Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
The Abpromise guarantee

Abpromise™承诺保证使用ab47351于以下的经测试应用

\"应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

Sandwich ELISA Use at an assay dependent concentration. 说明 Sandwich ELISA
Use at an assay dependent concentration. 图片 IL-1 beta Human ELISA Set (ab47351)

发表研究结果有使用 ab47351？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab47351 尚未被引用在任何文献中。

Thank you for contacting Abcam.

The antibodies of ab47351 are both monoclonals, raised in mice.

If there is anything else I can help you with, please let me know.

Thank you for contacting us.

1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. Unfortunately, ELISA plates are not supplied.

2) n/a. If you require kits including plates I may recommend ab108865 and ab108867, respectively.

Click here (or use the following: https://www.abcam.com/Human-IL-1-beta-ELISA-Kit-ab108865.html).
Click here (or use the following: https://www.abcam.com/Human-IL-6-ELISA-Kit-ab108867.html).

3) We can recommend any polystyrene 1x96 well or 12x8 well plate suitable for ELISA.

4) Please find attached the protocols for ab48478 and ab47351. The suggested procedures may need some optimization depending on the plastic, matrix, standard range.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry.

For ab47351 you can use 96 well ELISA plates. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required. I am sorry wedo not sell these from Abcam, I can recommendto do an internet search to find some.

ab48487 is a CRLF2 antibody that I am sorry is no longer available. Could you confirm the product code?

I hope this will be helpful, and I look forward to hearing from you with regards to the second item.

Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES
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