Description F2H^®-Kit Basic allows studying interactions between GFP- and RFP-tagged proteins in live mammalian cells.

Specificity Bait: GFP-fusion protein Prey: RFP-fusion protein, mCherry-fusion protein Tested on cytoplasmic, nuclear, and mitochondrial proteins Cell line: BHK (baby hamster kidney)

Application Visualize protein-protein interactions inside cells Validate your pull-down results or Y2H hits in mammalian cells Identify interacting protein domains

Principle

• F2H cells (BHK origin) carry a GFP-anchoring platform in the nucleus
• GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H cells
• GFP-bait is recruited to the platform → bright green spot
• If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot
• If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence

ChromoTek GmbH开创了一类新的免疫学研究工具，该工具源自细胞生物学和蛋白质组学的单域骆驼抗体。ChromoTek成立于2008年，由Ulrich Rothbauer周边的一个研究团队从慕尼黑路德维希马克西米利安大学（LMU）分拆而来，现已迅速发展成为著名的创新试剂供应商和大型制药公司值得信赖的服务提供商。我们的管理团队结合了互补的技能和经验，形成了持续增长的基础。