Description Analyze and manipulate the intramolecular interaction between the N- and C-terminal domains of Androgen Receptor (AR) with ChromoTek Fluorescent-Two Hybrid (F2H^®) AR. Wild-type (wt), as well as several mutant AR forms can be investigated.

Specificity AR is expressed as two separate domains Bait: AR ligand binding domain (AR-LBD) fused to GFP Prey: AR N-terminal domain (AR-NTD) fused to RFP Bait and prey interact.

Applications Detect early steps of Androgen Receptor activation Real-time agonists and antagonists screening Visualized in live mammalian cells

Available AR variants AR-LBD: wt, W741L, F876L, T877A, F876L-T877A

Click on the left picture for a live cell analysis example Live-cell F2H imaging of reversible interactions between AR-LBD-GFP (green) and AR-NTD-RFP (red) in F2H-BHK cells

ChromoTek GmbH开创了一类新的免疫学研究工具，该工具源自细胞生物学和蛋白质组学的单域骆驼抗体。ChromoTek成立于2008年，由Ulrich Rothbauer周边的一个研究团队从慕尼黑路德维希马克西米利安大学（LMU）分拆而来，现已迅速发展成为著名的创新试剂供应商和大型制药公司值得信赖的服务提供商。我们的管理团队结合了互补的技能和经验，形成了持续增长的基础。